One person was taken to the Barrie hospital for assessment, three others were treated on scene after a fire in a guest room at the Quality Inn on Hart Drive.
Barrie Fire says the call came in around 11:45 Thursday night.
Flames were contained to the one room, though guests in the area of the fire were moved to another wing of the hotel.
The Ontario Fire Marshal has been called in to help determine the cause of the fire.
A damage total is not available.