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Damage is pegged at $750,000 in a fire at Muskoka Boat Rentals at Gravenhurst's Muskoka Wharf.
A nearby resident called 9-1-1 around 4 a.m. Wednesday to alert the fire department about the flames.
In a tweet, the fire service said the fire is believed to be suspicious.
The fire was contained to the building and there were no injuries.
The Fire Marshal has been contacted.