Ontario Provincial Police say the two women killed in a collision between a car and a train in Tottenham are aged 19 and 24.
The names of the victims are not being released until next-of-kin have been notified.
Police say first responders were called to the scene around 11 p.m. on Thursday at a railway crossing on the 5th Line of New Tecumseth.
There were five people in the car. A female passenger was pronounced dead at the scene. Four other people, including the male driver, were taken to a Toronto trauma centre with serious injuries. A second woman who was transported to the hospital later died of her injuries.
Police are asking any witnesses to contact investigators.